Generation of VSV-G pseudotyped retroviral vector, concentrated and efficient transition

摘要

(57) this [summary] application, biological cells of a mammal other than a wide variety of vector and, derived from a retrovirus envelope glycoprotein of retrovirus, was replaced by the G glycoprotein of vesicular stomatitis virus It is intended to disclose when that transfer the exogenous gene, the use of such vectors. Furthermore, a method of generating retroviral vectors high titers are also disclosed, stable recombinant host cell line harboring a retroviral vector intended without envelope protein are thereby provided. Production of retroviral vectors high titer is initiated by introducing into the cell line a nucleic acid encoding a membrane-associated protein functionality. Can be concentrated to a titer of more than 10 ⁹ cfu / ml by ultracentrifugation, these are particularly useful in attempts of human gene therapy, a vector is disclosed in the present application includes an envelope protein of a retrovirus It is also possible to infect cells such as fish cells and hamster usually resistant to infection by the vector.