GENERATION CONCENTRATION AND EFFICIENT TRANSFER OF VSV-G PSEUDOTYPED RETROVIRAL VECTORS

摘要

The present application discloses retrovirus-derived vectors in which theretroviral envelope glycoprotein has been replaced by the Gglycoprotein of vesicular stomatitis virus, and the use of these vectors inthe transfer of exogenous genes into the cells of a wide variety ofnon-mammalian organisms. Also disclosed is a method for the generation ofretroviral vectors in high titers, wherein a recombinant, stablehost cell line is provided which harbors the retroviral vector of interestwithout envelope protein. High-titer retroviral vector productionis initiated by introducing nucleic acid encoding a functional membrane-associated protein into the cell line. The vectors disclosed in thepresent application can be concentrated by ultracentrifugation to titersgreater than 10 9 cfu/ml which are especially useful in human genetherapy trials, and can also infect cells, such as hamster and fish cells,that are ordinarily resistant to infection with vectors containing theretroviral envelope protein.