The magA gene where the code it does the protein which is joined to

摘要

PURPOSE: To obtain a new gene coding for a protein bound to an organic membrane covering magnetic fine particles produced in a specific magnetic bacterium and providing protein-bound magnetic fine particles useful in the medical or fermentation fields. etc., without requiring the practice of treatment such as immobilization. ;CONSTITUTION: This new isolated and purified magA gene codes for a protein bound to an organic membrane covering magnetic fine particles produced in a microbial cell of a magnetic bacterium AMB-1 and comprises a DNA sequence represented by the formula. The magnetic fine particles having the organic membrane, covering the surfaces thereof and bound to an enzyme, an antibody or other useful proteins useful in the medical or fermentation fields can be obtained simply by culturing transformed magnetic bacterium. separating the magnetic fine particles produced in the microbial cell without requiring the practice of treatment such as immobilization. The magA gene is obtained by transducing a transposon Tn5 which is a metastatic gene having a medicine- resistant factor into the magnetic bacterium AMB-1, then preparing a variant strain deficient in the productivity of the magnetic fine particles and cloning the gene.;COPYRIGHT: (C)1996,JPO