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Micro-particle array analysis system, micro-particle array kit, and chemical analysis method

机译:微粒阵列分析系统,微粒阵列试剂盒和化学分析方法

摘要

There is provided means for analyzing organism-related molecules, dealing with multi item analysis, that are captured according to probe species, and for collecting according to the probe species. A magnetic micro-particle array is fixed with magnets that are configured with magnetic micro-particles in a capillary and with an array of glass beads to which DNA probes of different types from each other are immobilized. A syringe pump and a cross valve are operated to circulate a sample solution in the magnetic micro-particle array, which is reacted with probe DNAs on a glass bead with a probe. Subsequently, a washing solution is introduced to wash inside of the capillary. Next, respective beads are measured for fluorescence intensities. Furthermore a particular bead is collected based on results of fluorescence measurement. Target molecules captured on a surface of the collected bead may be separated by heat-denaturation, which then may be subjected to next analysis.
机译:提供了用于分析与生物有关的分子,进行多项目分析,根据探针种类捕获并根据探针种类进行收集的手段。磁性微粒阵列用磁体固定,该磁体由毛细管中的磁性微粒和玻璃珠阵列构成,在玻璃珠上固定有彼此不同类型的DNA探针。操作注射泵和交叉阀以使样品溶液在磁性微粒阵列中循环,该溶液与带有探针的玻璃珠上的探针DNA反应。随后,引入清洗溶液以清洗毛细管内部。接下来,测量各个珠的荧光强度。此外,基于荧光测量的结果收集特定的珠。捕获在收集的珠子表面上的靶分子可以通过热变性分离,然后可以进行下一步分析。

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