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Method of affinity purifying proteins using modified bis-arsenical fluorescein
Method of affinity purifying proteins using modified bis-arsenical fluorescein
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机译:使用修饰的双砷荧光素亲和纯化蛋白质的方法
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摘要
The present invention features methods for purifying polypeptides of interest using a modified Fluorescein arsenical helix binder (FlAsH) compound immobilized on a solid support. An exemplary FlAsH target sequence motif is also presented. Examples of modification of the FlAsH compound which allow immobilization to a solid support are also provided. The present invention also provides DNA constructs for producing a dual affinity tagged polypeptide and methods for purification thereof.
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