Cell fixation and use in phospho-proteome screening

摘要

A new method of cell fixation using a mild denaturing heat and the fixative PermiFlow™ or similar fixative that preserves cell morphology, light scatter profiles, nucleic acid content, cell surface epitopes and unmasks internal epitopes previously not available. The method can be combined with analysis of each of these parameters by flow cytometry and thus has application in drug screening and patient or tumor monitoring protocols.