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High-throughput turbidometric assay for screening inhibitors of protein disulfide isomerase activity
High-throughput turbidometric assay for screening inhibitors of protein disulfide isomerase activity
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机译:高通量浊度分析法筛选蛋白二硫键异构酶活性抑制剂
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摘要
Methods for performing an end-point assay of protein disulfide isomerase activity. The method may be based on the enzyme-catalyzed reduction of insulin in the presence of dithiothreitol; measuring the aggregation of reduced insulin chains at 650 nm; and using hydrogen peroxide as a stop reagent.
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