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Genotyping by liquid chromatographic analysis of short nucleic acid fragments
Genotyping by liquid chromatographic analysis of short nucleic acid fragments
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机译:通过液相色谱分析短核酸片段进行基因分型
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摘要
The present invention provides genotyping analysis by liquid chromatographic analysis of short nucleic acid fragments. The nucleic acid fragments are amplification products using specifically designed oligonucleotides as primers and target nucleic acids containing nucleotides of interest as templates. The oligonucleotides contain recognition sequences for restriction endonucleases that cleave outside the recognition sequences. The short nucleic acid fragments can be rapidly and reliably analyzed using liquid chromatography, optionally followed by mass spectrometry, and the nucleotides of interest identified.
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