Use of the techniques voltamu00e9tricas for the study of the mechanism of cleavage of the DNA

摘要

"The use of techniques for the study of the mechanism of voltamu00e9tricas cleavage of DNA.Embodied in the form of a method voltamu00e9trico for elucidation of the mechanism of the reaction of cleavage of a double oligodesoxiribonucleotu00eddeo tape 19 mer catalyzed by zinc metaloenzima nucleaIf mung bean.A mechanism of the type S ~ n ~ 2 together with the participation of the three zinc ions in the active site of the nuclease mung bean (Zn1, Zn2, and zn3) in the reaction of cleavage of the oligo double ribbon has been proposed.In this mechanism, the hydrolysis of the connections fosfodiu00e9steres occurs with inversion of configuration at the phosphorus atom, forming a transitional state pentacoordenado,Which is stabilized by an arginine. An aspartic acid, binding of Zn2, helps to guide the nucleu00f3filo for the reaction.The ion Zn2, in addition to providing nucleu00f3filo (catalytic function), activates the trace by means of its coordination to the free oxygen of the phosphate of the phosphodiester bond is hydrolyzed.The ions Zn1 and zn3, in addition to stabilize the tertiary structure of the nuclease mung bean functions (structural), bind to the group abandonador during the cleavage reaction.And the same reacts with the transition state formed and promotes the reversal reaction.