Processes for obtaining biflavonoids, photoprotective, antioxidant, anti-inflammatory and anti-aging activities and their use in pharmaceuticals, nutraceuticals and cosmetics

摘要

"PROCEDURES FOR OBTAINING BIFLAVONOIDS, PHOTOPROTECTOR, ANTIOXIDANT, ANTIINFLAMMATORY AND ANTI-VENELEMENT ACTIVITIES AND THEIR USE IN DRUGS, NUTRACUES AND COSMETICS". This invention relates to processes for obtaining a biflavonoid fraction from Araucaria angustifolia leaves, photoprotective, antioxidant, anti-inflammatory and anti-aging activities and their use in the manufacture of cosmetics and medicaments useful in photoprotection, antioxidants, anti-inflammatory and anti-aging. More specifically, one of the processes for obtaining the biflavonoid fraction comprises the following steps: a) collecting fresh parts of the Araucaria angustifolia plant; b) drying the parts of Araucaria angustifolia in an oven at a temperature ranging from 30 to 70 ° C; c) milling the dried plant in a Tigre knife mill to a particle size of 2 to 6 mesh; d) macerating the obtained powder at a temperature between 30 and 70Â ° C with ethanol or any solvent of similar polarities for 2 to 6 days; e) preparing the extract by filtering the solution obtained by macerating with filter paper followed by concentration under reduced pressure at a temperature of 40 sym 20 198 C; down to 1/3 of the initial volume; f) precipitation of the biflavonoid fraction by addition of 1 to 3 L of water to the extract obtained, followed by evaporation at a temperature between 30 and 70 ° C, at reduced pressure to remove the solvent; g) centrifuging or decanting the resulting precipitate to separate the biflavonoid fraction from the supernatant, followed by drying of the solid obtained at 25 20Â ° C; h) characterization of the bifiavonoid fraction by high performance liquid chromatography, high performance liquid chromatography coupled with mass spectrometry, planar chromatography and nuclear magnetic resonance; i) identification of bifiavonoid fraction components using established standards as a comparison.