ATTENUATED RECOMBINANT BOVINE HERPESVIRUS I-VIRUS, VACCINE BASED THEREON, AND METHOD FOR DIFFERENTIATING ANIMALS INFECTED WITH THE RECOMBINANT VIRUS VACCINE

摘要

A safe live recombinant virus as well as a vaccine is produced by the deletion of a portion of the native glycoprotein E(gE) coding region of bovine herpesvirus 1(BHV-1) followed by the insertion of a plasmid including a foreign functional beta -gal at the gE locus. The deletion of gE gene stably attenuates the virus and serves as an immunological marker differentiating vaccinated animals from infected animals. Moreover, production of beta -gal allows easy assessment of gE DELTA 3.1IBR beta virus replication in vaccinated animals and serves as a phenotypic marker which distinguishes it from wild type virus replication in infected animals.