METHOD OF CRYOPRESERVATION OF ORGAN CULTURE OF THE THYROID GLAND OF NEW-BORN PIGLETS

摘要

A method of cryopreservation of organ culture of the thyroid gland of new-born piglets involves incubation in a cryopreservation solution containing the medium 199 and the 7% dimexide as cryoprotector, freezing up to -196 DEGREE C, keeping in liquid nitrogen and warming up. The incubation of organ culture is carried out at 22 DEGREE C for 30 minutes, and the freezing is carried out above the mirror of liquid nitrogen with the speed of 85-100 DEGREE C/min for 3 minutes with the further submerging into the liquid nitrogen.