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METHOD FOR ISOLATING FOREST CELLS OF OSTEOKLASTS AND INDUCING THEIR DIFFERENTIATION INTO EAST LOADS.
METHOD FOR ISOLATING FOREST CELLS OF OSTEOKLASTS AND INDUCING THEIR DIFFERENTIATION INTO EAST LOADS.
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机译:分离破骨细胞的森林细胞并将其分化为东部负荷的方法。
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摘要
This invention relates to: a method for differentiating osteoclast precursor cells (preosteoclasts) into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cells; a method for isolating preosteoclasts, which comprises culturing peripheral blood or joint fluid in the absence of cytokine for 1 to 3 weeks; an preosteoclasts, which is obtainable by the above method; a method for differentiating the preosteoclasts obtained by the above method into osteoclasts, which comprises culturing the preosteoclasts in the absence of accessory cell; an osteoclast, which is obtainable by the above method; a method for screening agents for treating metabolic bone diseases, which comprises using the preosteoclasts or the osteoclasts as described above; and agents for treating metabolic bone diseases, which is obtainable by the above screening method.
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