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PROCEDURE FOR OBTAINING HUMAN DNA FROM GINGIVAL CREVICULAR FLUID.

机译:从牙龈龈沟液中获取人类DNA的程序。

摘要

Procedure for obtaining human DNA from gingival crevicular fluid. It carries out the extraction of human DNA, for its analysis by molecular detection, as well as for the analysis of the genetic susceptibility to any disease, without affecting the activity of Taq polymerase in the PCR, obtaining a product of correct visualization after its electrophoresis in agarose gel and by staining with ethidium bromide, as well as subject to digestion with restriction enzymes (RFLP) for allelic study or to its direct sequencing, with diagnostic quality, based on careful preparation of gingival crevicular fluid by means of tips or strips of absorbent paper or an interdental brush, preventing contamination of the sample with the operator's own cells.
机译:从牙龈沟液中获取人类DNA的程序。它可以进行人类DNA提取,通过分子检测进行分析,以及对任何疾病的遗传易感性进行分析,而不会影响PCR中Taq聚合酶的活性,从而在电泳后获得正确可视化的产物在琼脂糖凝胶中进行染色,并用溴化乙锭染色,并用限制性酶(RFLP)消化以进行等位基因研究,或对其进行直接测序,具有诊断质量,其方法是基于通过尖端或条带精心制备牙龈沟液吸收性纸或齿间刷,以防止样品被操作员自己的细胞污染。

著录项

  • 公开/公告号ES2188384B1

    专利类型

  • 公开/公告日2005-01-01

    原文格式PDF

  • 申请/专利权人 CLINICA PERIO S.L.;

    申请/专利号ES20010001441

  • 发明设计人 MORILLO VELAZQUEZ JUAN MANUEL;

    申请日2001-06-21

  • 分类号C12Q1/68;C12N15/10;B01D21/26;G01N33/487;

  • 国家 ES

  • 入库时间 2022-08-21 22:15:00

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