DNA or fragments thereof, encoding the human erythropoietin, vectors, host cells or cell lines, DNAc or fragments thereof, encoding the human erythropoietin, human recombined erythropoietin, processes for obtaining human erythropoietin and human recombined erythropoietin, pharmaceutical composition and process for obtaining thereof

摘要

The present invention relates to biotechnology, medical and biological industry, medicine and, particularly to cloned DNA, encoding human erythropoietin, expressions of the indicated DNA and in vitro obtaining of active human erythropoietin. of DNA genome fragments from human fetal liver and DNAc fragments, providing exceptionally high levels of the erythropoietin expression into the mammal cells, it is obtained the cell line of the Chinese hamster ovary CHO, producing recombined EPO (CHO EPO Cla 4a4.02-7). As a result of the DNA cloned fragments expression into the mammal cells there was obtained the O-glycosylated EPO, having a specific activity of at least 200000 units/mg and the pharmaceutical composition, wherein the above-mentioned recombined EPO is used as active ingredient.The result of the invention consists in levels growth of the active human erythropoietin expression in vitro as well as in removal of adverse reaction during treatment with human recombined erythropoietin.