Method of identifying microorganisms based on DNA amplification.

摘要

Microorganism identification method based on DNA amplification.; The method comprises (i) obtaining patterns RAPD (random amplified DNA) of said microorganism, and (ii) comparing said patterns with a set of RAPD patterns representative of different microorganisms at the species level, wherein obtaining said RAPD patterns comprises DNA contact of the microorganism with a mixture for the enzymatic amplification of DNA comprising one or more initiators with a length between 18 and 30 nucleotides each, in a concentration of at least 0.1 uM of each them, and designed based on rDNA sequences; and wherein the annealing is performed at a temperature between 5 ° C and 15 ° C below the temperature of primer annealing. Application in the detection and identification of microorganisms.