USE OF MODIFIED NUCLEOTIDES IN 5 'IN MOLECULAR BIOLOGY AND IN MEDICINE.

摘要

Method for the detection of nucleic acids, which includes the incorporation of compounds of the general formula (I): in which: B means a nucleobase, W and Z mean in each case OR1, SR1, N (R1) 2 or R1, representing R1 in each case, independently, at each occurrence, hydrogen or an organic radical, X means OR2, SR2 or B (R2) 3, meaning R2 in each case, independently, hydrogen, a cation, or an organic radical, Y means NR3 or S, representing R3 hydrogen or an organic radical, and R means hydrogen, a cation, an organic radical or an optionally modified phosphate or diphosphate group, in nucleic acids and the subsequent specific dissociation for a nucleic acid site.