5'-MODIFIED NUCLEOTIDES AND THE APPLICATION THEREOF IN MOLECULAR BIOLOGY AND MEDICINE

摘要

The invention relates to 5'-modified nucleotides and to nucleic acids which contain these nucleotides. Processes for incorporating the 5'-modified nucleotides into nucleic acids, and the subsequent site-specific cleavage of the nucleic acids at the 5'-modified monomer building blocks, are also disclosed. These processes can be employed for nucleic acid sequencing, for generating nucleic acid libraries, for detecting mutations, for preparing support-bound nucleic acids and for pharmaceutical purposes.