Method of diagnosis of foot and mouth disease and the diagnostic kit

摘要

The present invention provides a method for diagnosing foot-and-mouth disease virus infection, comprising the steps of applying a predetermined amount of a test sample to a loading region of a strip; coupling a detection reagent including a given labeling reagent to an analyte of interest in the test sample to form a complex therebetween; developing the complex onto a wicking membrane; and observing changes in appearance of a reactivity zone having at least more than one immobilized phase selected from antigen, antibody or hapten on the predetermined region of the wicking membrane, derived from FMDV or obtainable from FMDV through an immunological reaction to determine the presence or absence of foot-and-mouth disease virus infection. It also provides a kit for diagnosing foot-and-mouth disease virus infection comprising a strip 1 including a reactivity zone 13 containing at least more than one immobilized phase selected from antigen, antibody or hapten thereon, derived from FMDV or obtainable from FMDV through an immunological reaction, and a control zone 14 for confining normal operation of the kit, provided on a predetermined region of a wicking membrane 9 ; and a housing 20 protecting the strip 1 from a variety of contaminants, and including at least a test sample application port 2 and an indicia window 4 for observing results of reaction in the reactivity zone 13 and the control zone 14 on the strip.