A DETECTION METHOD OF HLA COMPLEMENT DEPENDENT CYTOTOXIC ANTIBODIES BASED ON ENZYME-LINKED IMMUNOSORBENT ASSAY AND THE KIT THEREOF

摘要

The present invention provides a method of detecting HLA complement-dependent cytotoxic antibodies by enzyme-linked immunosorbent assay in vitro and the kit thereof. The reaction system comprises fixed HLA antigens or target cells comprising HLA antigens and liquid complements or antibodies against the complements, which were labeled with enzymes. The CFAbs in the samples to be determined will combine with the fixed HLA antigens or the HLA antigens on target cells and at the same time fix the enzyme-labeled complements in the reaction system or combine as an enzyme-labeled antibodies against the complements with the complement in the HLA antigen-CFAbs complexes, and then adding the corresponding substrates of the enzymes to ensure the color development or luminous reaction; the presence or absence or the relative levels of the CFAbs in the samples will be determined by detecting the optical density, because the optical density is corresponding to the CFAbs contents in the samples. Adding antibodies against characteristic marker (e.g. CD et al.) of the cell surface to the reaction system based on cells to recognize and isolate specific cell panels, can detect specifically the CDC reactions of the cell panels.