Homogeneous enzymatic assay for vitamin B6 and improvements in H2S detection

摘要

Enzymatic methods to determine the concentration of pyridoxal 5'-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular disease. The assay can be a homogeneous assay using the ability of PLP to function as a co-enzyme for homocysteinase and related enzymes and measuring the products of the reaction preferably spectrophotometrically. The invention also includes improvements in sensitivity of assays for measuring hydrogen sulfide production by measuring fluorescence as opposed to absorbance of the oxidized product of H2S with N, N-dialkyl p-phenylene diamine.