A FORMULATION AND A PROCESS FOR REDUCING THE DETERIORATION OF BLOOD WITH PARTICULAR REFERENCE TO DECREASE IN THE LEVEL OF 2,3 DIPHOSPHOGLYCERATE DURING STORAGE

摘要

CPDA (citrate-phosphate-dextrose-adenine) solution is the most widely used anti-coagulant preservative solution for collection and storage of whole blood In blood Bags. Even though the permitted period of storage of blood In CPDA solution in 35 days, there is a steady and progressive deterioration in the quality of the blood which increases with storage time. Some of most harmful changes are a drastic decrease in the level of 2,3 diphosphoglycerate (which is essential for delivery of oxygen to the tissues), increased hemolysis (which releases Hb and K+ from the RBCs into the plasma, both of which are harmful) and progressive solubilization of di-(2-ethyl hexyl) phthalate (DEHP), the plasticizer commonly used in the blood Bags which is also harmful. The present process reduces the deterioration of blood, more particularly due to reduction in 2,3 diphosphoglycerate during storage of blood, by means of formulation of anticoagulant preservative used in the process which prevents the extent of these changes in the blood during storage, particularly the drastic decrease in 2,3 DPG.