Lactic Acid Reduces LPS-Induced TNF-a and IL-6 mRNA Levels Through Decreasing IκBa Phosphorylation

摘要

cqvip:This study explored the effects over time of lactic acid (LA) on I?Bα phosphorylation and nuclear factor-kappa B (NF-?B) p65 protein expression, and on tumor necrosis factor a (TNF-α) and interleukin-6 (IL-6) mRNA levels in rat intestinal mucosa microvascular endothelial cells (RIMMVECs) stimulated by lipopolysaccharide (LPS). I?Ba, phosphorylated I?Ba (p-I?Ba) and p65 protein levels were monitored by Western blot analysis, and TNF-a and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF-a and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on I?Ba phosphorylation to prevent activation of NF-?B was after 6 h. These results suggest that LA reduces TNF-a and IL-6 mRNA levels through decreasing I?Ba phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF-?B.