MULTIPLEXED ASSAYS USING ELECTROPHORETICALLY SEPARATED MOLECULAR TAGS

摘要

By using releasable plurality of molecular tags that are coupled to binding compounds specific for the analyte of interest, the present invention provides methods and algorithms for determining one or more analytes in a sample to. After that binds specifically to the analyte to their respective binding compound, to form a complex, molecular tags of binding compounds that form such complexes, are released and disconnected. However, the molecular tags of binding compounds that do not form such complexes is not released. Are then electrophoretically separated along the electrophoretic standard of more than one, generate an electropherogram data, the identity of each molecular tag, molecular tags are released, the electric one or more It is determined by the position of the peak in its corresponding data against such migration standards. Thus, due to factors such as differences in variability devices, assay conditions, reagents, strain in the electropherogram data can be explained.