METHODS FOR PRODUCING FUNCTIONAL, SINGLE-CHAIN Fv ANTIBODY FRAGMENTS ON THE SURFACE OF BACTERIOPHAGE PARTICLES

摘要

we identify a member of a specific binding pair (sbp) for expressing the dna encoding a colony of genetic diversity of the members of pbs in recombinant host cells in which the members of pbs are functional form on the surface of a core of m genetic recombination degree of secreted (rgdp) containing dna encoding a member of pbs or polypptidique thereof, through the expression of its constituent member of pbs or polypeptide as a fusion with a component of the rgdp packaging. you can select according to the affinity with pbs and pbs member of complementary dna of rgdp selected for the expression of selected members of pbs.it is thus possible to obtain anticorporaux sbp members whose channels are expressed, one of which is fused to the packaging and the other is free to form an association to polypeptide fusion partner. can be used as a phagemide fusion expression vector and packaging contributes to the phagemide dna encapsidation.using this method, we can combine dna libraries encoding the respective channels of multimeric sbp members, in order to obtain pbs with greater genetic diversity than that which would be easily using the traditional methods.