ANALYSIS METHODS USING BIOMARKERS CONCENTRATED WITH BIOMARKERS ATTRACTANT MOLECULES

摘要

A method for discovering biomarkers by examining the molecules that adhere to and are concentrated by larger biomarker attractant molecules is disclosed. Also disclosed is a method for diagnosing biological states based on biomarkers bound to one or more biomarker attractant molecules obtained from one or more biological fluids. In a particular embodiment, molecules bound to high abundance proteins with a molecular weight above the kidney filtration cutoff (about 45 kDa) are collected from serum and compared between groups of subjects to detect biomarkers. In another embodiment, artificial materials such as nanoparticles are introduced into the bloodstream of different groups of subjects and allowed to harvest smaller molecules. The harvested molecules may then be compared between the groups to detect new biomarkers. Once discovered, biomarkers that bind to one or more particular biomarker attractant molecules may be used to provide a diagnosis.