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SINGLE MOLECULE SPECTROSCOPY FOR ANALYSIS OF CELL-FREE NUCLEIC ACID BIOMARKERS
SINGLE MOLECULE SPECTROSCOPY FOR ANALYSIS OF CELL-FREE NUCLEIC ACID BIOMARKERS
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机译:单分子光谱分析无细胞核酸生物标志物
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摘要
The present invention relates, e.g., to a method for detecting a nucleic acid molecule of interest in a sample comprising cell-free nucleic acids, comprising fluorescently labeling the nucleic acid molecule of interest, by specifically binding a fluorescently labeled nanosensor or probe to the nucleic acid of interest, or by enzymatically incorporating a fluorescent probe or dye into the nucleic acid of interest, illuminating the fluorescently labeled nucleic acid molecule, causing it to emit fluorescent light, and measuring the level of fluorescence by single molecule spectroscopy, wherein the detection of a fluorescent signal is indicative of the presence of the nucleic acid of interest in the sample.
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