Method for producing purified tocotrienols and tocopherols using liquid chromatography

摘要

A method separating tocotrienols from tocopherols and the isomers thereof on a large-scale or commercially feasible basis. Separation is accomplished using reverse phase partition liquid chromatography. The chromatography column is prepared using a stationary phase comprising a lipophilic reverse phase chromatography media. The mobile phase consists of a solvent which capable of solubilizing the crude substrate, tocotrienols and tocopherols. The chromatography column containing the stationary and mobile phases is loaded with a crude substrate and the tocotrienols, tocopherols and/or isomers thereof are eluted at a predetermined linear velocity.