PCR-BASED MONITORING IN WASTEWATER BIOTREATMENT SYSTEMS

摘要

Microorganism monitoring for the based on PCR in the system of biological wastewater treatment. Specifically, both abundance and the expression of instruction/effector or the assortment of genes are determined, wherein the expression of " effect " gene is relevant to a kind of degrading activity of specific microbiological specimens, and the abundance of " instruction " gene is relevant to the abundance of microorganism. The expression of effector is measured by competitive quantitative RT-PCR, and the abundance of indicator is measured by competitive quantitative PCR. The indicator and effector can be same or different gene. In one embodiment, the indicator and effector are the sequence from glyphosate oxidoreductase (gox) gene, and the quantitative technique has used competitive quantitative PCR and competitive quantitative RT-PCR.