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PLANT SYSTEM FOR COMPREHENSIVE GENE FUNCTION ANALYSIS WITH THE USE OF FULL-LENGTH cDNA

机译:利用全长cDNA进行基因功能综合分析的植物系统

摘要

A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function comprises: (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants having the cDNA introduced thereinto; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.
机译:容易确定表型的基因起因,因此全面分析了基因功能。一种分析基因功能的方法,包括:(a)用全长cDNA文库感染植物群,该全长cDNA文库含有表达调控序列以将cDNA引入植物中; (b)从导入了cDNA的植物中选择符合期望选择要求的植物; (c)从所选植物中分离cDNA; (d)将如此分离的cDNA重新导入与导入了该cDNA的植物相同种类的植物中,以根据选择要求重新确定表型。

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