TELOMERE PROTEIN TRF2 DNA-BINDING DOMAIN MUTANT PROTEIN, TELOMERE DNA MUTANT AND UTILIZATION OF COMPLEX STRUCTURE OF TRF2 DNA-BINDING DOMAIN WITH DOUBLE-STRANDED DNA

摘要

A TRF2 DNA-binding domain mutant protein represented by the following (a) or (b) or its salt. (a) A protein having an amino acid sequence derived from the amino acid sequence of wild type hTRF2 DNA-binding domain by at least one substitution selected from among the substitution of lysine at the 10-position into arginine, the substitution of alanine at the 34-position into serine, the substitution of alanine at the 47-position into serine and the substitution of arginine at the 59-position into lysine. (b) A protein having an amino acid sequence derived form the amino acid sequence of the protein of the above (a) by deletion, substitution or addition of one to several amino acids other than the amino acid at the 10-position, the amino acid at the 34-position, the amino acid at the 47-position and the amino acid at the 59-position, and being superior in the ability to binding to a double-stranded DNA containing a sequence represented by 5’-TTAGGG-3’ to wild type TRF2 DNA-binding domain having the amino acid sequence represented by SEQ ID NO:2.