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CONTINUOUS-FLOW ENZYME ASSAY WITH MASS SPECTROMETRY DETECTION
CONTINUOUS-FLOW ENZYME ASSAY WITH MASS SPECTROMETRY DETECTION
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机译:质谱检测连续流动酶
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摘要
The present invention relates to an on-line detection method comprising the steps of: contacting an effluent of a fractionation step with a controlled amount of an enzyme; allowing the enzyme to interact with analytes suspected to be present in the effluent; addition of a controlled amount of a substrate for said enzyme; allowing a reaction of the enzyme with the substrate providing one or more modified substrate products; and detection of unreacted substrate, or a modified substrate product using a mass spectrometer.
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