METHOD FOR THE ELIMINATION OF INTERFERENCES IN IMMUNOCHROMATOGRAPHIC ASSAYS

摘要

A method and device for carrying out immunoassays in which non analytespecific binding of heterophilic antibodies to a labeled antibody in a captureregion produces an incorrect measure of the amount of an analyte attached tothe antibody. Immunoglobulin from the same animal source as the labeledantibody is added to the sample fluid to prevent non-specific binding of theheterophilic antibodies in the capture region. One part of specific bindingpair is added to said antibody or its label capable of binding to a secondpart of the binding pair immobilized in a control region downstream of saidcapture region for trapping the portion of the labeled anti-body which is notbound to the analyte. Preferably said binding pair is biotin/avidin orfluoroscein/anti-fluoroscein.