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METHODS FOR THE ENRICHMENT OF LOW-ABUNDANCE POLYNUCLEOTIDES
METHODS FOR THE ENRICHMENT OF LOW-ABUNDANCE POLYNUCLEOTIDES
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机译:低丰度多核苷酸的富集方法
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摘要
The invention relates to methods for the selective enrichment of low-abundance polynucleotides in a sample. These methods use enzymatically non-extendable nucleobase oligomers to selectively block polymerase activity on high abundance species, thereby resulting in an enrichment of less abundant species in the sample. The invention also relates to the pools of enriched polynucleotides produced by the methods. The resulting pools of enriched polynucleotides find a variety of uses, including the analysis of gene expression and the creation of cDNA libraries.
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