SCREENING METHOD OF ANTIFUNGAL AGENT WHICH INHIBITS CHROMOSOME SEPARATION IN FUNGI TO INHIBIT THEIR CELL DIVISION AND THE ISOLATED ANTIFUNGAL AGENT USING THE SAME

摘要

PURPOSE: A screening method of an antifungal agent and the isolated antifungal agent using the same are provided, thereby easily screening the antifungal agent which inhibits chromosome separation in fungi to inhibit their cell division. CONSTITUTION: The screening method of an antifungal agent comprises the steps of: providing a transformed host cell containing centrosome DNA of Saccharomyces cerevisiae or DNA having a 90% homology to the same centrosome DNA, a hybrid promoter with a galactose-inducible promoter, and a lacZ reporter gene; contacting a test compound with the transformed host cell; and determining whether the transcription of the reporter gene is inhibited, wherein the host cell is Saccharomyces cerevisiae(KCTC 10481BP) or Candida glabrata.