A RAPID DNA EXTRACTION METHOD FOR PCR-BASED ANALYSIS OF TRANSGENIC FISH

摘要

The present invention relates to a method for quickly the transgenic fish and extract of good quality DNA that can be used in PCR to organizations of trace from small transgenic fish to economic analysis, dodecyl tissue collected from fish room sodium sulfate; DNA comprising the step of culturing in a basic buffered solution containing (sodium dodecyl sulfate SDS) and neutralized by addition of a buffer solution containing a non-ionic surface active agent in the culture medium and recovering the supernatant after centrifuging them According to the extraction method, it is possible to quickly secure a small amount of template DNA that can be used for PCR from fish fin 20 minutes, and so at the same time provides a good template for PCR DNA with no difference at all and the purified DNA, which is required by the prior art DNA complex separation process, expensive removal of the protein enzyme and the reaction time can not be required over the time Well, the time required for the release of transgenic fish prior analysis, we can significantly reduce the costs and efforts.