Method for Improving a Genetic Stability for the Insert in +-Single-Stranded RNA Virus Recombinant Vectors

摘要

The present invention is a single chain RNA virus as a recombinant vector to a method to improve the genetic stability of the inserted sequence, and more particularly, foreign to be inserted to create a vaccine by using a single-stranded RNA viral vector, in particular polio virus vector DNA method for using the absence PCR method synthesize inserted DNA sequence of 500 bp in length without the need for a template-binding reaction a G / C volume of distribution of the sequence to the above object comprising the steps of mutation be uniformly distributed throughout the foreign DNA sequence It relates to a method of increasing the genetic stability of the recombinant live vaccine using the poliovirus vector containing the. The method of the invention provides a method to significantly improve the genetic stability of the service conventional polio virus vector in the manufacture of a live vaccine methods, which may include a variety of antigenic determinant sites than recombinant live vaccine by introducing a vaccine gene, so the development of recombinant live vaccines polio virus vector, and to improve significantly the usefulness of the single-stranded RNA virus recombination vector for.