A method for culturing isolated microspore of pepper

摘要

The present invention relates to a sopoja culture of pepper and pepper and then blending a medicine collected from buds viewed by vortexing naked the sopoja and, to remove the somatic tissue debris by centrifugation at a naked sopoja and only harvesting sopoja, the harvest to the tooth shape on the sopoja a pre-culture medium for 3 days at 30~32 ℃, and after the pre-treatment the sopoja a tooth shape at a density of 80,000~100,000 microspore / ㎖ NLN medium for 3-4 weeks culture in the dark state 25~27 ℃ and it is characterized in that the obtaining times.; The present invention it is possible to obtain a very large quantity of boat merely when callus from sopoja of single cells haploid as well as the study of the differentiation of embryonic development and cell haploid breeding, sopoja mutation, sopoja transgenic including plant biotechnology It can be used in very useful.