METHOD FOR PRODUCING L-subculture BRUCELLA ADORTUS STRAIN AND 206

摘要

1. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 l - u0432.abortus subcultures, including multiple u043fu0430u0441u0441u0438u0440u043eu0432u0430u043du0438u0435 u0431u0440u0443u0446u0435u043bu043b on nutritional environment containing u043du0430u0442u0440u0438u00a0 u043cu0430u0433u043du0438u00a0 chloride, sulfate, sucrose, u0433u043bu0438u0446u0435 rin, normal horse serum and u0431u0438u0446u0438u043bu043bu0438u043d - 3 in the growing u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0u0445, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 that use strain.abortus and 206, with high u0430u043du0442u0438u0433u0435u043du043du043eu0439 activity and high antibiotic resistance u043fu0435u043du0438u0446u0438u043bu043bu0438u043du043eu0432u043eu0433u043e u0440u00a0u0434u0430 and u043fu0438u0442u0430u0442u0435u043bu044cu043du0430u00a0 environment additionally contains liver the infusion and agar, with the following components: ratio of mas.;agar 1.2;u043du0430u0442u0440u0438u00a0 chloride 0.1;u043cu0430u0433u043du0438u00a0 sulfate 0.05;sucrose 10.0;glycerin 1.0;u043du043eu0440u043cu0430u043bu044cu043du0430u00a0 u043bu043eu0448u0430u0434u0438u043du0430u00a0 serum 10.0;liver infusion.;and u0431u0438u0446u0438u043bu043bu0438u043d - 3 u0434u043eu0431u0430u0432u043bu00a0u044eu0442 in breeding ground in the growing u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0u0445: 10 - 50 - 100 - 1000 - 2000 - 3000 - 4000 - 5000 - 6000 - 7000 to 8000 - 9000 iu / ml medium.;2. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the u0434u043bu00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 l subculture u0432.abortus and 206 with a characteristic in response to a single injection to rabbits the incomplete antibodies in the reaction u0432u044bu00a0u0432u043bu00a0u0435u043cu044bu0435 u043au0443u043cu0431u0441u0430 in u0442u0438u0442u0440u0435 1: 400, strain, u043fu0430u0441u0441u0438u0440u0443u044eu0442 to the final concentration u0431u0438u0446u0438u043bu043bu0438u043du0430 - 3 2000 iu / ml medium.;3. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that the u0434u043bu00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 l subculture u0432.abortus and 206 with a characteristic in response to a single injection to rabbits the specific u0430u0433u0433u043bu044eu0442u0438u043du0438u043du044b, u0432u044bu00a0u0432u043bu00a0u0435u043cu044bu0435 in response u0430u0433u0433u043bu044eu0442u0438u043du0430u0446u0438u0438 in u0442u0438u0442u0440u0435 1: 1600, strain, u043fu0430u0441u0441u0438u0440u0443u044eu0442 to the final concentration u0431u0438u0446u0438u043bu043bu0438u043du0430 - 3 9000 iu / ml medium.