METHOD AND SYSTEM FOR PREPARING tissue samples for histological and pathological examination

摘要

1.method for freezing of biochemically active tissue and frozen tissue sample includes immersion cooling environment, u0446u0438u0440u043au0443u043b u0438u0440u043eu0432u0430u043du0438u0435 cooling environment around the sample tissue in essentially u043fu043eu0441u0442u043eu00a0u043du043du043eu0439 specified speed and temperature u0434u043bu00a0 u0437u0430u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0 sample tissue soto u0432u0438u0442u0440u0438u0444u0438u0446u0438u0440u043eu0432u0430u043bu0441u00a0 tissue sample and the tissue sample u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 biochemically active after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0, time u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u0435 samples of tissue and study u0440u0430u0437u043cu043eu0440u043eu0436u0435u043du043du043eu0433u043e sample tissue.;2. method for preparing 1, further an outlet sample tissue.;3. method for 1, in which the study sample u0440u0430u0437u043cu043eu0440u043eu0436u0435u043du043du043eu0439 tissue includes a histological study.;4. method for 1, in which the study u0440u0430u0437u043cu043eu0440u043eu0436u0435u043du043du043eu0433u043e sample tissue includes u0443u043bu044cu0442u0440u0430u0441u0442u0440u0443u043au0442u0443u0440u043du043eu0435 study.;5. method for 1, in which the study includes the use of u0438u043cu043cu0443u043du043eu0433u0438u0441u0442u043eu0445u0438u043cu0438u0447u0435u0441u043au043eu0433u043e u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0.;6. way to p.5, which includes fluorescent antibodies in immunohistochemistry staining.;7. method for 1, in which more than about 55 per cent of the sample tissue showed a lack of anatomical damage of cellular structure and u043eu0441u0442u0430u044eu0442u0441u00a0 biochemically active after the u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;8. method for 1, in which more than about 45 per cent of the sample tissue showed a lack of u043fu043eu0432u0440u0435u0436u0434u0435u043du0438u00a0 cell anatomical structure and u043eu0441u0442u0430u044eu0442u0441u00a0 biochemically active after the u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;9. method for 1, in which more than about 85 per cent of the sample tissue u0441u043eu0445u0440u0430u043du00a0u044eu0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u044eu0442u0441u00a0 intact after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;10. method for 1, in which the cooling environment support at a temperature of from about - 20 degrees to about 30 degrees u0446u0435u043bu044cu0441u0438u00a0 u0446u0435u043bu044cu0441u0438u00a0.;11. method for 1, in which the speed of cooling environment around the sample tissue u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 around 35 liters per minute on foot cooling environment in area not more than employment about 24 inches in width, and 48 inches in depth.;12. method for 1, in which the coolant circulating through the engine, and the node with the environment u043au0440u044bu043bu044cu0447u0430u0442u043au043eu0439, steeped in the cooling environment.;13. method for u0446u0438u0440u043au0443u043bu0438u0440u043eu0432u0430u043du0438u0435 1, further a cooling environment around u043cu043du043eu0433u043eu0437u0430u0445u043eu0434u043du043eu0433u043e coils attached to the heat exchanger, steeped in the cooling environment serpentine heat exchanger capable of u0443u0434u0430u043bu00a0u0442u044c, with at least the same amount of heat from the cooling environment, a u043eu0445u043bu0430u0436u0434u0430u044eu0449u0430u00a0 wednesday u0443u0434u0430u043bu00a0u0435u0442 of sample tissue.;14.method of preparing tissue samples u0434u043bu00a0 u0438u0441u043fu043eu043bu044cu0437u043eu0432u0430u043du0438u00a0 u0434u043bu00a0 u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0 by immersion of the biologically active tissue in the cooling medium have, and direct freezing of tissue to a temperature greater than about 30 degrees u0446u0435u043bu044cu0441u0438u00a0 by u0446u0438u0440u043au0443u043bu0438u0440u043eu0432u0430u043du0438u00a0 cooling environment around the u0437u0446u0430 tissue, with essentially u043fu043eu0441u0442u043eu00a0u043du043du043eu0439 specified speed and temperature,what u0432u0438u0442u0440u0438u0444u0438u0446u0438u0440u0443u0435u0442u0441u00a0 tissue sample, the tissue sample u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e maintains its structure and u043eu0441u0442u0430u0435u0442u0441u00a0 biochemically active after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;15. method for preparing on p.14, further cuts the sample tissue.;16. a way to question 14, further providing some samples of tissue.;17. a way to question 14, in which the study includes histological study.;18. a study on p.14, which includes u0443u043bu044cu0442u0440u0430u0441u0442u0440u0443u043au0442u0443u0440u043du043eu0435 study.;19. a study on p.14, which includes the use of u0438u043cu043cu0443u043du043eu0433u0438u0441u0442u043eu0445u0438u043cu0438u0447u0435u0441u043au043eu0433u043e u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0.;20. way on p.19, which includes fluorescent antibodies in immunohistochemistry staining.;21. a way to question 14, in which more than 40 per cent of the sample tissue u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 biochemically active after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;22. a way to question 14, in which more than 80 per cent of the sample tissue u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 biochemically active after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;23. a way to question 14, in which more than about 85 per cent of the sample tissue u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 intact after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;24. a way to question 14, in which the cooling environment support at a temperature of about 20 degrees to about 30 degrees u0446u0435u043bu044cu0441u0438u00a0 u0446u0435u043bu044cu0441u0438u00a0.;25. a way to question 14, in which the speed of cooling environment around the sample tissue u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 around 35 liters per minute on foot cooling environment through the area of no more than an eye luo 24 inches in width, and 48 inches in depth.;26. a way to question 14, in which the coolant circulating through the engine, and the node with the environment u043au0440u044bu043bu044cu0447u0430u0442u043au043eu0439, steeped in the cooling environment.;27. method for u0446u0438u0440u043au0443u043bu0438u0440u043eu0432u0430u043du0438u0435 p.14, further a cooling environment around u043cu043du043eu0433u043eu0437u0430u0445u043eu0434u043du043eu0433u043e coils attached to the heat exchanger, steeped in the cooling medium serpentine heat exchanger capable of u0443u0434u0430u043bu00a0u0442u044c, with at least the same amount of heat from the cooling environment, a u043eu0445u043bu0430u0436u0434u0430u044eu0449u0430u00a0 wednesday u0443u0434u0430u043bu00a0u0435u0442 of sample a swatch and.;28.the system u0434u043bu00a0 u0438u0441u043fu043eu043bu044cu0437u043eu0432u0430u043du0438u00a0 during preparation of sample tissue u0434u043bu00a0 u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0, u0441u043eu0434u0435u0440u0436u0430u0449u0430u00a0 reservoir with a cooling medium, is the possibility of receiving u043eu0431u0440u0430u0437u0446 a biochemically active tissue u0434u043bu00a0 u043fu043eu0433u0440u0443u0436u0435u043du0438u00a0 in cooling environment, one or more devices u0434u043bu00a0 u0446u0438u0440u043au0443u043bu00a0u0446u0438u0438 cooling environment of u0434u043bu00a0 u0446u0438u0440u043au0443u043bu0438u0440u043eu0432u0430u043du0438u00a0 decree anna cooling environment.coil heat exchanger u0434u043bu00a0 u0443u0434u0430u043bu0435u043du0438u00a0 heat from the cooling environment, freeze the knot by the heat of the heat exchange coils attached to u0434u043bu00a0 u0443u0434u0430u043bu0435u043du0438u00a0 nick and the reservoir u0434u043bu00a0 cooling environment, the one or more u0446u0438u0440u043au0443u043bu00a0u0446u0438u043eu043du043du044bu0445 device and the node are not u0434u043bu00a0 freeze a u0437u0430u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0 sample tissue temperatures higher.what about - 30 degrees u0446u0435u043bu044cu0441u0438u00a0 by u0446u0438u0440u043au0443u043bu0438u0440u043eu0432u0430u043du0438u00a0 cooling environment around the sample tissue, essentially u043fu043eu0441u0442u043eu00a0u043du043du043eu0439 a given speed and temperature so that u0440u0430u0437u0435u0446 u0432u0438u0442u0440u0438u0444u0438u0446u0438u0440u0443u0435u0442u0441u00a0 tissue, the tissue sample u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 biochemically active after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;29. system, p.28, in which the study includes histological study.;30. the system includes u0443u043bu044cu0442u0440u0430u0441u0442u0440u0443u043au0442u0443u0440u043du043eu0435 p.28, in which the research study.;31. system, p.28, in which the study includes the use of u0438u043cu043cu0443u043du043eu0433u0438u0441u0442u043eu0445u0438u043cu0438u0447u0435u0441u043au043eu0433u043e u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0.;32. the system includes p.31 in which immunohistochemistry staining fluorescent marked antibodies.;33. system, p.28, in which more than 40 per cent of the sample tissue u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 biochemically active after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;34. system, p.28, where more than 80 per cent of the sample tissue u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 biochemically active after u0440u0430u0437u043cu043eu0440u0430u0436u0438u0432u0430u043du0438u00a0.;35. system, p.28, in which more than about 85 per cent of the sample tissue u0441u043eu0445u0440u0430u043du00a0u0435u0442 its u0430u043du0430u0442u043eu043cu0438u0447u0435u0441u043au0443u044e structure and u043eu0441u0442u0430u0435u0442u0441u00a0 intact.;36. system, p.28, in which cooling environment support at a temperature from about 20 to about 30 degrees u0446u0435u043bu044cu0441u0438u00a0.;37. system, p.28, in which the speed of cooling environment around the sample tissue u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 around 35 liters per minute on foot cooling environment in area not more than okay. are 24 inches in width, and 48 inches in depth.;38. system, device u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 u0446u0438u0440u043au0443u043bu00a0u0446u0438u043eu043du043du043eu0435 p.28, in which a node with an engine and u043au0440u044bu043bu044cu0447u0430u0442u043au043eu0439, immersed in a cooling environment.;39. system, p.28, in which coolant circulating around the heat exchanger coils attached to the environment u043cu043du043eu0433u043eu0437u0430u0445u043eu0434u043du043eu0433u043e, steeped in the cooling environment, and the coil u0442u0435u043fu043bu043eu043eu0431u043c u0443u0434u0430u043bu00a0u0442u044c u0435u043du043du0438u043au0430 capable of at least the same amount of heat from the cooling environment, a u043eu0445u043bu0430u0436u0434u0430u044eu0449u0430u00a0 wednesday u0443u0434u0430u043bu00a0u0435u0442 of sample tissue.