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Tissue microarrays in pathological examination of apoptotic acinar cells induced by dexamethasone in the pancreas of rats with severe acute pancreatitis

机译：地塞米松诱导的重症急性胰腺炎大鼠胰腺凋亡腺泡细胞的组织芯片检测

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摘要

BACKGROUND:The good therapeutic effects of large dose of dexamethasone on severe acute pancreatitis (SAP) patients have been proved. This study was designed to investigate the inlfuence of dexamethasone on apoptosis of acinar cells in the pancreas of rats with SAP and the protein expression of the apoptosis-regulating genes Bax and Bcl-2. METHODS: Ninety Sprague-Dawley rats with SAP were randomly divided into a model group and a dexamethasone treated group (45 rats in each group), and another 45 rats formed the sham operation group. Survival rates were calculated and gross pathological changes in the pancreas of each group were observed under a light microscope 3, 6 and 12 hours after operation. Tissue microarray technology was applied to prepare pancreatic tissue sections. The changes in Bax and Bcl-2 protein expression levels of pancreatic tissues from each group were assessed by immunohistochemical staining, and TUNEL staining was used to evaluate changes in apoptosis index. RESULTS: The model and treated groups did not differ in mortality at each time point. The pathological score for the pancreas in the treated group was signiifcantly lower than that in the model group at 3 and 6 hours. The positive rates of Bax protein expression in the head and tail of the pancreas in the treated group at all time points were all markedly higher than those of the model group. The positive rate of Bcl-2 protein expression in the head of the pancreas in the treated group was signiifcantly higher than that of the model group at 3 hours. TUNEL staining showed that the pancreas head and tail apoptosis indices of the treated group were markedly higher than those of the model group after 6 hours. CONCLUSIONS: Apoptosis may be a protective response to pancreatic cell injury. The mechanism of action of dexamethasone in treating SAP may be related to the apoptosis of acinar cells in the pancreas induced by apoptosis-regulating genes such as Bax and Bcl-2. The advantages of tissue microarrays in pathological examination of the pancreas include saving of time and energy, efifciency and highly representative.
机译：背景：已证明大剂量地塞米松对严重急性胰腺炎（SAP）患者具有良好的治疗作用。本研究旨在探讨地塞米松对SAP大鼠胰腺腺泡细胞凋亡的影响以及凋亡调控基因Bax和Bcl-2的蛋白表达。 方法：将90只SAP鼠Sprague-Dawley大鼠随机分为模型组和地塞米松治疗组（每组45只），另45只大鼠组成假手术组。在手术后3、6和12小时，在光学显微镜下计算存活率并观察每组胰腺的总体病理变化。组织微阵列技术被用于制备胰腺组织切片。通过免疫组织化学染色评价各组胰腺组织Bax和Bcl-2蛋白表达水平的变化，并用TUNEL染色评价细胞凋亡指数的变化。 结果：模型组和治疗组的死亡率在每个时间点均无差异。在3和6小时时，治疗组的胰腺病理评分显着低于模型组。在所有时间点，治疗组的胰头和尾中Bax蛋白表达的阳性率均显着高于模型组。 3小时后，治疗组胰头中Bcl-2蛋白表达的阳性率显着高于模型组。 TUNEL染色显示6小时后，治疗组的胰头和尾细胞凋亡指数明显高于模型组。 结论：凋亡可能是对胰腺细胞损伤的保护性反应。地塞米松治疗SAP的作用机制可能与凋亡调控基因如Bax和Bcl-2诱导的胰腺腺泡细胞凋亡有关。组织微阵列在胰腺病理检查中的优势包括节省时间和精力，效率高且具有代表性。

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  • 来源
    《国际肝胆胰疾病杂志（英文版）》 |2007年第005期|527-536|共10页
  • 作者

    Xi-Ping Zhang; Hua Tian; Bei Lu; Li Chen; Ru-Jun Xu; Ke-Yi Wang; Zhi-Wei Wang; Qi-Hui Cheng; Hai-Ping Shen;

  • 作者单位

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

    Department of General Surgery Zhang XP and Lu B;

    Department of Pathology Xu RJ;

    Central Laboratory Wang KY and Wang ZW;

    Department of Gynaecology and 0bstetrics Cheng QH;

    and 0perating Room Shen HP, Hangzhou First People's Hospital, Hangzhou 310006, China;

    and Department of General Surgery, Second Afifliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China Tian H and Chen L;

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  • 入库时间 2022-08-19 03:39:25
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