RECOMBINANT PLASMID DNA pHINSO5 ENCODING HYBRID POLYPEPTIDE COMPRISING HUMAN PROINSULIN, ESHERICHIA COLI CELL TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS05 AND STRAIN OF BACTERIUM ESCHERICHIA COLI JM109/pHINS05 AS PRODUCER OF HYBRID POLYPEPTIDE COMPRISING HUMAN PROINSULIN

摘要

FIELD: genetic engineering, proteins.;SUBSTANCE: recombinant plasmid DNA pHINS05 comprises gene encoding hybrid protein consisting of N-terminal fragment of human gamma-interferon, peptide linker His₄GlySerArg and human proinsulin. E. coli cells are transformed with recombinant plasmid DNA pHINS05 and the strain E. coli JM109/pHINS05 is obtained that is a producer of hybrid protein with human proinsulin. Invention provides increasing yield of recombinant human proinsulin and to reduce cost of the technological process in preparing human insulin.;EFFECT: valuable biological properties of plasmid DNA and strain.;4 cl, 2 dwg, 4 ex