Optimizing detection of prions including a pathological form in body fluid or homogenized tissue, precipitates prion protein which is made visible by addition of dye bonded to substrate

摘要

The prion protein PrP is precipitated from a sample of the body fluid or of the homogenized tissue and is made visible by addition of a dye bonded to a substrate : The dye substrate is a pectin, e.g. an amylopectin. The prion protein is made visible by formation of a correspondingly-colored pellet on the base of the reaction vessel by centrifugation in the presence of the dye-marked substrate. The PrP rendered visible, is then investigated for the existence of the pathological form PrPSc, in an ELISA (enzyme-linked immunosorbent assay), a western blot, a conformation dependent immunoassay (CDI) or another prion detection method.