Apparatus for carrying out a polymerase chain reaction or reverse transcriptase polymerase chain reaction, comprises that it measures electrical conductivity simultaneously

摘要

An instrument measures electrical conductivity simultaneously with a polymerase chain reaction (PCR) or reverse transcriptase polymerase chain reaction (RT-PCR). An instrument measures electrical conductivity simultaneously with a PCR or RT-PCR. Microelectrodes are built into the reaction vessel employed, and a computer analyses the measured values. The microelectrodes are integrated into the vessel or into individual wells of a microtitration plate. Contacts are provided for them. The temperature in each vessel is also measured and e.g. a Peltier effect heater/cooler is employed. During the PCR, parameters are measured, e.g. electrical conductivity, temperature, cycle number and time for appropriate correlation. During optional melt curve analysis, electrical conductivity is measured.