Analytical method for determining the predisposition a patient on at least one disease, as well as suitable amplification for such a method

摘要

The invention concerns a method for analysing a patient's genetic predisposition to at least one disease. It also concerns an amplification process adapted to said method. The method consists in bringing a liquid sample containing at least one type of amplicons, derived from the amplification of at least one polymorphous region of interest related to the searched disease(s), in the presence of probes selected as follows: at least one specific typing probe, said to be low-resolution, capable of being hybridized on the polymorphous region of interest of at least one gene or one group of alleles of said gene borne by the amplicon and associated with at least said disease(s); and at least a specific subtyping probe, said to be high-resolution, capable of being hybridized on said polymorphous region of interest of the allele or group of alleles of the specific typing probe, said to be low-resolution, the high-resolution probe(s) enabling to discriminate the allele(s) associated with the susceptibility and/or the allele(s) associated with resistance to said disease(s), on the basis of their hybridization or non-hybridization. The invention is particularly applicable in the field of diagnosis.