首页>
外国专利>
'cort' - pcr assay to distinguish between endogenous reverse transcriptase activity in eukaryontischen cells from contamination by infectious retroviruses
'cort' - pcr assay to distinguish between endogenous reverse transcriptase activity in eukaryontischen cells from contamination by infectious retroviruses
展开▼
机译:“ cort”-pcr分析法可区分真核细胞中内源性逆转录酶活性与传染性逆转录病毒的污染
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to the detection of the presence of reverse transcriptase enzymatic activity in eukaryotic cell lines. Specifically, the present invention comprises a method for discriminating between endogenous reverse transcriptase activity in a substrate cell line (e.g., avian) used as substrates for the manufacture of biological products (including viral vaccines) from exogenous infectious retrovirus by using a combination of transmissibility assay (viral replication) and PBRT assay (RT-PCR). Appropriate levels of viral amplification and number of PCR cycles are determined and employed that permit detection of exogenous infectious RT activity but not endogenous RT activity.
展开▼