Protein glycosylation modifications in (Pichiapastoris) Pichia pastoris

摘要

Including Pichia pastoris and in particular the genus Pichia, and a strain of methylotrophic yeast which is genetically engineered, the present invention provides a strain capable of producing a protein with a modified glycosylation or reduced. Methods of producing glycoproteins with glycosylation using Pichia strains Such genetically engineered, modified and / or reduced are also provided. Vector containing a sequence or collapse OCH1 coding sequence, about mannosidase II α-1,2- mannosidase I, glucosidase II, and GlcNAc transferase I are also encompassed by the present invention to transform a methylotrophic yeast. A kit for providing a vector that the intended also included in the present invention.