Biological specificity of two-photon excitation fluorescence detection method and apparatus for the

摘要

(57) biospecific reagent is labeled microparticles as [Abstract] bioaffinity binding solid phase, a fluorescent label, and using a fluorescence detection system based on two-photon fluorescence excitation, the analyte, labeled reagent, and the fluorescence emitted by the particles from the individual particles when suspended randomly the particles through the focal volume of the laser beam simultaneously contacted the solid phase, and focuses the two-photon excitation laser beam into the reaction suspension method of monitoring the real-time dynamics measurement method and of the end point of the biological affinity reaction suspension in biological fluids and to measure the signal. In this method, the monitored kinetically the signal to obtain information about the concentration of the analyte before the reaction is to be close to the highest point of the response. The growth rate of the signal strength is directly proportional to the concentration of the analyte, the concentration of the analyte can be expected in the initial stage of the reaction. That by the monitoring of the growth rate, it is whether will continue the reaction is more than the highest point of the response curve the concentration of the analyte, and whether higher than the binding capacity of the reagent is also predicted. According to this method, it can be completely eliminated the risk of obtaining ambiguous results in the measurement of the low and high concentrations. It is also possible that the dynamic range of the assay, to extend considerably. By combining the features of the present invention, Fast and has a large dynamic range for the samples of small volume is very fluid, a biological specimen or other cell suspensions, a single step, the separated unnecessary and biospecific method and apparatus for performing a sex test can be performed.