Synthetic peptides useful in biological essays for detecting infections caused by group O HIV-1 viruses

摘要

Synthetic peptides of the monomer type with 13 to 33 amino acids, in linear form or in a form cyclized by means of inter-cysteine disulphide bridges, have the general formula (I): Δ-Z-TrpGlyCys-Θ-CysTyrThrSer-Ω  (I) wherein Δis a biotinyl radical, a biocytinyl radical, a hydrogen atom, an acetyl (CH₃CO—) radical, an aliphatic chain which may contain one or two thiol, an aldehyde functional group, or an amine functional group, Z represents peptide sequence -₁-Ser-₂-, -₁-Gln-₂-, or -₁-Asn-₂-, wherein -₁ represents a peptide sequence of 0 to 9 amino acids and -₂ represents a peptide sequence of 0 to 5 amino acids, Θis -Arg Gly Arg Leu Ile-(SEQ ID NO: 15), -Arg Gly Arg Leu Val-(SEQ ID NO: 16), -Arg Gly Lys Leu Ile-(SEQ ID NO: 17), -Arg Gly Lys Leu Val-(SEQ ID NO: 18), -Lys Gly Arg Leu Ile-(SEQ ID NO: 19), or -Lys Gly Arg Leu Val-(SEQ ID NO: 20), Ω, attached to the —CO— group of serine, is a hydroxyl (—OH) radical, an amino (—NH₂) radical, an alkoxy radical having 1 to 6 carbon atoms, a peptide sequence of formula Val-Σ-Ψ wherein Σ represents a sequence of formula -(AA₁)-Trp Asn-(AA₂)-(AA₃) wherein (AA₁) represents an amino acid different from lysine, (AA₂) represents an amino acid, and (AA₃) is serine or a threonine residue, and Ψ, attached to the —CO— residue of the free AA₃ amino acid, is OH, NH₂, or an alkoxy radical having from 1 to 6 carbon atoms, and a peptide sequence of formula -Val-Ψ wherein Ψ, attached to the —CO— residue of valine, is OH, NH₂, or an alkoxy radical having from 1 to 6 carbon atoms.