Method for amplifying nucleic acid and analysis of single-nucleotide polymorphism using the same

摘要

A method amplifies a nucleic acid by preparing an oligonucleotide being capable of complementarily hybridizing with a specific region of a target nucleic acid containing at least one mutation site, the oligonucleotide having at least one sequence being non-complementary to any of possible sequences of the at least one mutation site, subjecting the oligonucleotide to hybridization with the target nucleic acid, and carrying out a complementary-strand synthesis. A single-nucleotide polymorphism is analyzed using this method.